Journal: Investigative Ophthalmology & Visual Science

Article Title: Plasma Kallikrein Mediates Vascular Endothelial Growth Factor–Induced Retinal Dysfunction and Thickening

doi: 10.1167/iovs.15-18272

Figure Lengend Snippet: Effect of VEGF on plasma kallikrein system components in the retina. Western blot analyses of ( A ) plasma prekallikrein (PPK) and plasma kallikrein (PKal) and ( B ) high molecular weight kininogen (HK) in retinal extracts obtained from WT mice at 24 hours post intravitreal injections of VEGF (100 ng/eye) or PBS vehicle alone. Comparisons are * P < 0.05 and ** P < 0.01.

Article Snippet: Extracts were prepared from PBS-perfused retina in tissue protein extraction reagent (TPER; Thermo Scientific, Waltham, MA, USA) with protease inhibitor cocktail (no. P8340, Sigma-Aldrich) and analyzed by Western blotting using anti-KLKB1, plasma kallikrein (no. AF2498, R&D) and anti-high molecular weight kininogen antibody (no. ab1004; Abcam, Cambridge, MA, USA).

Techniques: Clinical Proteomics, Western Blot, High Molecular Weight